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0.05). The results also revealed that the accuracies of traditional method and PCR assays in the diagnosis of the disease were 81 %, and 100 respectively. There was moderate correlation (0.43) between both techniques by the Kappa (k) test. However, The PCR technique recorded the highest sensitivity (100%) and specify (100%) for A. marginale detection. In conclusion, by the findings of the present study, it has been confirmed for the first time that A. marginale is the causative agent of anaplasmosis of cattle in the study areas and the best technique for the detection of either acute or chronic cases in cattle was the PCR assay.]]>
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