Rebwar Ahmed Ahmed; Shakhawan Latif Mahmmod; Nawroz Akram Kakarash; Muhamad Omer Baba SHekh
Abstract
Theileria annulata has been identified as a major cause of bovine theileriosis in Iraq. The research was designed to reveal the prevalence of Theileriosis (Theileria annulata) in cattle in Sulaimani province, Kurdistan Region- Iraq from the 10th of March 2021 to the 10th of April 2021. ...
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Theileria annulata has been identified as a major cause of bovine theileriosis in Iraq. The research was designed to reveal the prevalence of Theileriosis (Theileria annulata) in cattle in Sulaimani province, Kurdistan Region- Iraq from the 10th of March 2021 to the 10th of April 2021. Eighty blood samples were collected. Different techniques of parasitic detection, including Microscopic and PCR amplification were used. The results of microscopic examination and PCR assay revealed that 27.5% and 31.25% cattle were infected, respectively. The microscopic examination of the Giemsa-stained blood smears of infected erythrocytes revealed the typical morphological structure of piroplasm. According to the phylogenetic analysis of the partial 18S ribosomal RNA gene, Theileria field isolates were shown to be related to other T. annulata strains, including those from Turkey, Italy, and Pakistan.This study concludes that T. annulata is the causative agent of theileriosis of cattle in Sulaimani province and suggests that PCR technique is excellent tools for epidemiological researches and control programs.
Shakhawan Latif Mahmmod; Rebwar Bahir Ahmed; Nawroz Akram Kakarash; Ihsan K Zangana; Mohammed Omar Baba Sheikh
Abstract
The goal of this study was to determine both the incidence of anaplasmosis (Anaplasma Marginale) and phylogenetic relationship between A. marginale isolates from cattle in Sulaimani province, Kurdistan Region- Iraq during (March 10th to April 10th 2021) and those from other Anaplasma spp. A total of ...
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The goal of this study was to determine both the incidence of anaplasmosis (Anaplasma Marginale) and phylogenetic relationship between A. marginale isolates from cattle in Sulaimani province, Kurdistan Region- Iraq during (March 10th to April 10th 2021) and those from other Anaplasma spp. A total of two isolates were tested for the major surface protein (msp4) gene for this purpose. Eighty blood samples of cattle (51 males and 29 females) were examined using both microscopic examination and PCR tests. Overall results were 23/80 (28.7 5%) and 8/80 (10 %) using microscopic examination and PCR assay, respectively. Age and sex were not significant factors in the appearance of infection, since no statistically significant difference in infection rate has been observed among sex and age group of cattle (P value >0.05). The results also revealed that the accuracies of traditional method and PCR assays in the diagnosis of the disease were 81 %, and 100 respectively. There was moderate correlation (0.43) between both techniques by the Kappa (k) test. However, The PCR technique recorded the highest sensitivity (100%) and specify (100%) for A. marginale detection. In conclusion, by the findings of the present study, it has been confirmed for the first time that A. marginale is the causative agent of anaplasmosis of cattle in the study areas and the best technique for the detection of either acute or chronic cases in cattle was the PCR assay.
