Document Type : Original Article

Authors

• Sally Khalil Baqer
• Nzar Ali Ameen Shwan

MLT department, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq

Abstract

Abstract
Type 2 Diabetes (T2D) is considered the most common form of diabetes; it develops when pancreatic cells produce an excess amount of insulin, which leads to insulin resistance by peripheral cells. T2D is an illness caused by interactions between genetics and environmental factors. Genetic factors are involved in the evolution of T2D, and several genetic variants increase the risk of developing T2D. The SNPs rs1801282 in the PPARG gene and rs9939609 in the FTO gene were found to be associated with increasing the risk of T2D in different populations. We aimed to discover if rs1801282 in the PPARG gene and rs9939609 in the FTO gene are responsible for increasing the risk of T2D in the Kurdistan population. In the current study, DNA from 200 unrelated samples (100 T2D and 100 non-diabetic control) individuals were genotyped using Allele-specific PCR for both SNPs. The PCR methods were validated by the Sanger sequencing method.
The association analysis for the rs1801282 variant (adjusted by sex, age, and BMI) showed significant differences between the case and the control groups; individuals with genotypes (GG and GC) had a higher risk of the disease (p-value = 0.0045, OR = 3.96, 95%CI: 1.31-11.94) than genotype (CC). On the contrary, there were no significant differences (p-value= 0.39) between the case and control groups for the rs9939609 variant.
Our finding suggested that the variant rs1801282 in the PPARG gene was a suspectable SNP in T2D in the Kurdish population, while SNP rs9939609 in the FTO gene was not associated with T2D. Further investigations with larger number of samples are required to validate our findings.

Keywords

• Keywords: T2D
• Allele-specific PCR
• Association study
• SNPs
• FTO
• PPARG

Main Subjects

• Biology