Document Type : Original Article


1 College of Medicine, University of Garmian, Kalar, Iraq

2 Department of Medical Science, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

3 Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran


In this study, gold nanoparticles were fabricated using two different functional groups such as crown ether and carboxylic acid and evaluated as a sensor to detect copper ions in an aqueous solution. The Au-crown/carboxylic is characterized with TEM, FTIR spectroscopy. To compare the response of the sensor to copper ions, the UV-vis spectrum was characterized before and after the increase of Cu2+ ions. The mechanism to be considered for measuring copper ions in aqueous solutions is that Cu2+ ions can successfully complexation with Au-crown/carboxylic which comes with color changes from red to blue. In the range of 75 nM to 1250 nM, the link between the decrease in adsorption intensity and the concentration of Cu2+ ions at A630/A545 is linear with 0.9814 and 150 nM correlation coefficient and the limit of detection, respectively. In other words, among several other cations, the sensor assay especially measures copper ions and also has very simple and able to measure and detect copper ions in an aqueous solution.


  1. References

    1.  Cay S, Uyanık A, Özaşık A (2004) Single and binary component adsorption of copper (II) and cadmium (II) from aqueous solutions using tea-industry waste. Sep Purif Technol 38 (3):273-280
    2. Tian Y, Wu M, Liu R, Li Y, Wang D, Tan J, Wu R, Huang Y (2011) Electrospun membrane of cellulose acetate for heavy metal ion adsorption in water treatment. Carbohydr Polym 83 (2):743-748
    3. van den Berg CM (2006) Chemical speciation of iron in seawater by cathodic stripping voltammetry with dihydroxynaphthalene. Anal Chem 78 (1):156-163
    4. Huber JK (1999) Determination of Cu, Fe, Mn, and Zn in blood fractions by SEC-HPLC-ICP-AES coupling. Analyst 124 (5):657-663
    5. Arnold GL, Weyer S, Anbar A (2004) Fe isotope variations in natural materials measured using high mass resolution multiple collector ICPMS. Anal Chem 76 (2):322-327
    6. Andersen JE (2005) A novel method for the filterless preconcentration of iron. Analyst 130 (3):385-390
    7. Zhao W, Brook MA, Li Y (2008) Design of gold nanoparticle‐based colorimetric biosensing assays. ChemBioChem 9 (15):2363-2371
    8. Wang G, Wang Y, Chen L, Choo J (2010) Nanomaterial-assisted aptamers for optical sensing. Biosens Bioelectron 25 (8):1859-1868
    9. Han MS, Lytton‐Jean AK, Oh BK, Heo J, Mirkin CA (2006) Colorimetric Screening of DNA‐Binding Molecules with Gold Nanoparticle Probes. Angew Chem Int Ed 45 (11):1807-1810
    10. Zhu X, Liu Y, Yang J, Liang Z, Li G (2010) Gold nanoparticle-based colorimetric assay of single-nucleotide polymorphism of triplex DNA. Biosens Bioelectron 25 (9):2135-2139
    11. Al Shehab S, Patra D (2021) Binding of metal ions to the Curcumin mediated methoxy polyethylene glycol thiol conjugated greenly synthesized gold nanoparticles: A fluorescence spectroscopic study. Journal of Photochemistry and Photobiology A: Chemistry407:113083
    12. Zhang F, Liu J (2021) Label‐Free Colorimetric Biosensors Based on Aptamers and Gold Nanoparticles: A Critical Review. Analysis & Sensing 1 (1):30-43
    13. Sreelekshmi P, Lakshmi SA, Babu G, Devika V, Rajeev N, Sadanandan S (2020) Peptide dendrimer stabilized gold nanoparticles as sensors. Materials Today: Proceedings 26:382-386
    14. Aili D, Selegård R, Baltzer L, Enander K, Liedberg B (2009) Colorimetric protein sensing by controlled assembly of gold nanoparticles functionalized with synthetic receptors. Small 5 (21):2445-2452
    15. Xu Y, Wang J, Cao Y, Li G (2011) Gold nanoparticles based colorimetric assay of protein poly (ADP-ribosyl) ation. Analyst 136 (10):2044-2046
    16. Meena R, Mehta VN, Bhamore JR, Rao PT, Park T-J, Kailasa SK (2020) Diaminodiphenyl sulfone as a novel ligand for synthesis of gold nanoparticles for simultaneous colorimetric assay of three trivalent metal cations (Al3+, Fe3+ and Cr3+). J Mol Liq 312:113409
    17. Li S, Liu X, Liu Q, Chen Z (2020) Colorimetric Differentiation of Flavonoids Based on Effective Reactivation of Acetylcholinesterase Induced by Different Affnities between Flavonoids and Metal Ions. Anal Chem 92 (4):3361-3365
    18. Karami C, Mehr SY, Deymehkar E, Taher MA (2017) Naked Eye Detection of Cr3+ and Co2+ Ions by Gold Nanoparticle Modified with Azomethine. Plasmonics:1-8
    19. Karami C, Taher MA (2017) Colorimetric Sensor of Cobalt Ions in Aqueous Solution Using Gold Nanoparticles Modified with Glycyrrhizic Acid. Plasmonics:1-9
    20. Zhu X, Yang H, Wang N, Zhang R, Song W, Sun Y, Duan G, Ding W, Zhang Z (2010) A facile method for preparation of gold nanoparticles with high SERS efficiency in the presence of inositol hexaphosphate. J Colloid Interface Sci 342 (2):571-574
    21. Karamipour S, Sadjadi M, Farhadyar N (2015) Fabrication and spectroscopic studies of folic acid-conjugated Fe 3 O 4@ Au core–shell for targeted drug delivery application. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 148:146-155
    22. Fotoohi B, Mercier L (2015) Some insights into thechemistry of gold adsorption by thiol and amine functionalized mesoporous silica in simulated thiosulfate system. Hydrometallurgy 156:28-39
    23. Garcia M, De la Venta J, Crespo P, Llopis J, Penadés S, Fernández A, Hernando A (2005) Surface plasmon resonance of capped Au nanoparticles. Physical Review B 72 (24):241403
    24. Lin S-Y, Liu S-W, Lin C-M, Chen C-h (2002) Recognition of potassium ion in water by 15-crown-5 functionalized gold nanoparticles. Anal Chem 74 (2):330-335
    25. Pearson RG (1963) Hard and softacids and bases. J Am Chem Soc 85 (22):3533-3539
    26. Alizadeh A, Khodaei M, Karami C, Workentin M, Shamsipur M, Sadeghi M (2010) Rapid and selective lead (II) colorimetric sensor based on azacrown ether-functionalized gold nanoparticles. Nanotechnology 21 (31):315503
    27. Pedersen CJ (1988) The discovery of crown ethers (Noble Lecture). Angew Chem Int Ed 27 (8):1021-1027
    28. Khan MN, Wahab MF (2007) Characterization of chemically modified corncobs and its application in the removal of metal ions from aqueoussolution. J Hazard Mater 141 (1):237-244
    29. Albrecht TWJ, Addai-Mensah J, Fornasiero D (2011) Effect of pH, concentration and temperature on copper and zinc hydroxide formation/precipitation in solution. Chemeca 2011: Engineering a Better World: Sydney Hilton Hotel, NSW, Australia, 18-21 September 2011:2100
    30. Zhang L-p, Xing Y-p, Liu C, Zhou X-h, Shi H-c (2015) Label-free colorimetric detection of Cu 2+ on the basis of Fenton reaction-assisted signal amplification with unmodified gold nanoparticles as indicator. Sensors and Actuators B: Chemical 215:561-567
    31. Guo Y, Wang Z, Qu W, Shao H, Jiang X (2011) Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles. Biosens Bioelectron 26 (10):4064-4069
    32. Alizadeh A, Khodaei MM, Hamidi Z, Bin Shamsuddin M (2014) Naked-eye colorimetric detection of Cu 2+ and Ag+ ions based on close-packed aggregation of pyridines-functionalized gold nanoparticles. Sensors and Actuators B: Chemical 190:782-791
    33. Karami C, Taher MA (2019) A novel enzyme-less amperometric sensor for hydrogen peroxide based on nickel molybdate nanoparticles. J Electroanal Chem 847:113219
    34. Baghayeri M, Veisi H, Farhadi S, Beitollahi H, Maleki B (2018) Ag nanoparticles decorated Fe 3O 4/chitosan nanocomposite: synthesis, characterization and application toward electrochemical sensing of hydrogen peroxide. Journal of the Iranian Chemical Society 15 (5):1015-1022
    35. Star BG, Shahlaei M, Karami C (2021) A novel fluorescent turn-on probefor hydrogen peroxide based on carbon dots. Journal of Materials Science: Materials in Electronics:1-9