Conventional and Molecular Diagnosis of Theileriosis (Theileria annulata) in Cattle in Sulaimani Province, Northern Iraq.

Ahmed, Rebwar Ahmed; Mahmmod, Shakhawan Latif; Kakarash, Nawroz Akram; Baba SHekh, Muhamad Omer

doi:10.24271/psr.26

Conventional and Molecular Diagnosis of Theileriosis (Theileria annulata) in Cattle in Sulaimani Province, Northern Iraq.

Document Type : Original Article

Authors

Rebwar Ahmed Ahmed ¹

Shakhawan Latif Mahmmod ²

Nawroz Akram Kakarash ³

Muhamad Omer Baba SHekh ⁴

¹ Department of Clinic and internal medicine, College of veterinary medicine , university of sulaimani

² Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani

³ University of Sulaimani, College of Veterinary Medicine

⁴ Molecular Diagnostic Laboratory, Sulaimani Veterinary Directorate, Sulaimani, Kurdistan Region- Iraq

10.24271/psr.26

Abstract

Theileria annulata has been identified as a major cause of bovine theileriosis in Iraq. The research was designed to reveal the prevalence of Theileriosis (Theileria annulata) in cattle in Sulaimani province, Kurdistan Region- Iraq from the 10^th of March 2021 to the 10^th of April 2021. Eighty blood samples were collected. Different techniques of parasitic detection, including Microscopic and PCR amplification were used. The results of microscopic examination and PCR assay revealed that 27.5% and 31.25% cattle were infected, respectively. The microscopic examination of the Giemsa-stained blood smears of infected erythrocytes revealed the typical morphological structure of piroplasm. According to the phylogenetic analysis of the partial 18S ribosomal RNA gene, Theileria field isolates were shown to be related to other T. annulata strains, including those from Turkey, Italy, and Pakistan.This study concludes that T. annulata is the causative agent of theileriosis of cattle in Sulaimani province and suggests that PCR technique is excellent tools for epidemiological researches and control programs.

Keywords

T. annulata

Tropical theileriosis

Blood smear

PCR

Sulaimani province

20.1001.1.27065944.2021.3.2.6.6

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