This study examines the synthesis of copper oxide nanoparticles (NPs) by using a Q-Switched Nd-Yag (1064 nm) laser on copper foil immersed in Distilled Water (DW). The solution color changed to light green refers to the production of copper oxide NPs. The generated nanoparticles were studied to determine their characteristics as a function of pulse laser shots and the NPs were obtained by taking 500 and 1000 laser pulse shots on Cu target. Thin films deposited on both (glass and silicon) substrates were characterized by X-Ray Diffraction (XRD), Field Emission-Scanning Electron Microscopy (FE-SEM), and Atomic Force Microscopy (AFM) techniques. Later, regarding the colloidal nanoparticles, NPs were instantly characterized by UV-vis spectroscopy and examined by TEM microscopy. The production rate of Cu-NPs concentration in the colloidal solution was measured by atomic absorption spectra type (ICP-OES), which increased by increasing the number of laser shots in the liquid volume. The Energy Dispersion Spectroscopy (EDS) analysis resulted in the presence of copper (Cu) and oxygen (O) elements in the film structure with a nearly stoichiometry ratio. The optical energy gap was decreased to (2.44 eV) with increasing the number of laser shots in the colloidal solution.