GC-Mass Spectormetry Profiling of Essential Oils from Aerial Parts of Hypericum triquetrifolium Turra using ITEX and HD Methods

Document Type : Original Article


Sulaimani Polytechnic University, Medical Laboratory Science (MLS) Department


Hypericum (H.) triquetrifolium contains numerous bioactive molecules in the aerial parts. Such molecules have numerous biological activities, including antioxidant, anti-inflammatory, antidepressant, antimicrobial, antifungal, antiviral, and anticonvulsant compounds. Despite the presence of H. triquetrifolium in Iraq, no previous studies have examined the biochemical contents of plant in the region. This study is carried out to evaluate the variability of the essential oil profile of aerial parts of Hypericum triquetrifolium Turra. Samples were also analyzed using gas chromatography-mass spectrometry (GC-MS) with two different extraction methods, namely in-tube extraction (ITEX) dynamic headspace and hydrodistillation (HD). A total of thirty-three, forty-three, and thirty-nine compounds were identified in stem, leaves, and flowers, respectively. Monoterpene hydrocarbons were the main compounds in the essential oil extracted from different parts. The highest constituent detected in the ITEX/GC-MS in stem parts was alpha-pinene (20.47%). On the other hand, the predominated compound of the essential oil detected by the HD method was cubenol (26.72%). The essential oil extracted from leaves by ITEX and HD methods showed 43 compounds. ITEX/GC-MS detected 32 products in the essential oil of leaves; vubenol was the highest (23.64%), and Using ITEX and HD/GC–MS, a subset of 39 chemical components in H. triquetrifolium essential oil were detected in the flower. Twenty-five out of 39 compounds, were analyzed by ITEX/GC-MS. The main constituent was 3-methyl-nonane (27.76%). HD/GC-MS method was characterized by detecting 23 components in flower oil. The major chemical structure identified in the essential oil in flower by HD/GC-MS was the monoterpene hydrocarbon represented by alpha-pinene (18.39%). Our data indicates that the ITEX method is more accurate than HD in the separation of essential oil components in this plant.


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