MnO, Mn:5%ZnO, and Mn:10%ZnO nanostructured thin films were deposited on glass substrates using chemical spray pyrolysis (CSP). To investigate their structural and optical properties, the synthesized thin films were characterized by XRD, FE-SEM, and UV-VIS spectrophotometer. X-ray diffraction demonstrated that deposition conditions affected crystallite size in these thin films, and Scherrer equation showed that the average crystalline size increased with ZnO doped manganese solution. The linear plot shows positive slope (0.0004 and 0.001) for all samples MnO and Mn:10%ZnO, which have a tensile strain and a lattice expansion in nano-tetragonal samples, except for Mn:5%ZnO, which has a negative slope (-0.0013). FE-SEM images showed that the particle size for all samples was 35.7, 56.9, and 23.6 nm for MnO, Mn:5%ZnO, and Mn:10%ZnO nanostructured thin films, respectively, which matches XRD analyses. Within the range of the visible spectrum, the optimal average transmission value falls between 20% and 60%. The Tauc relation was utilized to determine the optical energy-band gap (Eg), which exhibited an increase from 2.93 eV to 3.11 eV upon ZnO-doping. The Mn: ZnO thin films exhibit versatile optical properties that are significant for various applications, including transparent electromagnetic interference (EMI) shielding materials, photovoltaic, and solar cells. The optimal sample yielded an optical band gap of 3.06 eV for Mn:5%ZnO, which was observed to be lower than that of pure ZnO films, which had an optical band gap of 3.37 eV.