Document Type : Original Article

Authors

• Sahar Abdalkarim Salih ¹
• Rozhgar Abd Hussein ¹
• Imad Majeed Noori ²
• Shorsh Hussein Bapir ³
• Kareem Maulud Hamad ⁴
• Saber Hamad ^{5, 6}

¹ Department of Medical Laboratory Science, Technical College of Applied Science, Sulaimani Polytechnic University, Sulaymaniyah, Kurdistan Region, Iraq.

² Department of Nursing, Technical College of Health and Medical, Sulaimani Polytechnic University, Sulaymaniyah, Kurdistan Region, Iraq.

³ Department of Horticulture, College of Agricultural Engineering Sciences, University of Raparin, Rania, Kurdistan Region, Iraq

⁴ Department of Animal resource, College of Agricultural Engineering Sciences, University of Raparin, Rania, Kurdistan Region, Iraq.

⁵ Department of Field Crops and Medicinal Plants, College of Agricultural Engineering Sciences, Salahaddin University, Erbil, Kurdistan Region, Iraq

⁶ Department of Biology Education, Faculty of Education, Tishk International University, Erbil, Kurdistan Region, Iraq.

Abstract

The study was examined the impact of varying concentrations of hesperetin on the cryopreservation of rooster sperm. Sperm were diluted with a Lake Extender containing (0, 15, 20, 25, and 30) μM hesperetin. After a 30-second freeze-thaw operation at 37 °C, membrane integrity, sperm motility characteristics, aberrant morphology, apoptotic status, and mitochondrial activity were assessed. The concentration of 25 μM hesperetin observed the highest values (P < 0.01) were (64.26 %, 30.44 %, 35.88 mm/s, 21.77 mm/s, 61.08 mm/s, 35.66%, 61.14%, 16.23 Hz, 61.71%, 61.71%, and 61.33%) for (Total motility, progressive motility, average velocity path, curved velocity, straight-line velocity, linearity, straightness, beat cross frequency, membrane integrity, mitochondrial activity, and viability, respectively). In contrast, it had the lowest values (3.94mm, 13.67%, 13.30%, and 25.37%) recorded for lateral head displacement amplitude, total abnormality, apoptotic sperm, and dead sperm, respectively. It may be concluded that adding 25 μM hesperetin is an effective method for preserving the quality of cryopreserved rooster sperm.

Keywords

• hesperetin
• sperm
• rooster
• spermatozoa
• cryopreservation

Main Subjects

• Agriculture