Evaluation the Sensitivity of three Immunological Diagnostic Technique for the diagnosis of Toxoplasmosis

Document Type : Original Article

Authors

1 Department of Microbiology and Parasitology, College of Veterinary Medicine, University of Basrah.

2 Department of Parasitology and Microbiology, College of Veterinary Medicine, University of Basrah

10.24271/psr.2024.435812.1473

Abstract

Background: Toxoplasmosis is widely recognized as a highly widespread parasite infection in humans, capable of vertical transmission from mother to fetus. Clinical problems associated with toxoplasmosis during pregnancy include spontaneous miscarriage, premature labour, stillbirth, and foetal abnormalities.
Objective: to evaluate the sensitivity of three immunological methods for the diagnosis of T. gondii in serum and placental tissue of aborted women.
Materials and Methods:80 blood and placental Specimens were obtained from women who underwent abortion and were admitted to the Maternity ward and Child hospital in Kirkuk city. Blood samples analyzed for anti-Toxoplasma antibodies IgM and IgG using ELISA and Combo Rapid test and placental samples were tested for Toxoplasma gondii antigen in the placental tissue using Immunohistochemistry technique.
Results: The seropositivity of women who had abortions to T. gondii was 30% (IgG) when Rapid test used and 16.3% (IgG) when ELISA used. No Seropositivity of IgM showed in all cases in both rapid test and ELISA. 76.9% from total (13) seropositive cases in ELISA was positive when IHC used.
Conclusion: the finding of this study indicates that IHC is more sensitive immunological technique than serological Assay for the direct diagnosis of T. Gondii in the placental tissue of aborted women.

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